Zyxin Polyclonal Antibody

Applications

Tested Applications:

WB,ELISA

Species specificity:

human, mouse, rabbit

Positive WB detected in:

A549 cells, MCF-7 cells

Recommended dilution:

WB : 1:500-1:1000

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:

Zyxin fusion protein Ag0419

Full name:

zyxin

Calculated molecular weight:

80 kDa

Observed molecular weight:

78 kDa

Gene symbol

ZYX

Synonyms

ESP 2, HED 2, ZYX, zyxin, Zyxin 2

Background

Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin (ZYX) is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles.