Source: Prokaryotic expression 

Host: E.coli 

Residues: Pro644~Leu866 

Tags: N-terminal His and GST Tag 

Subcellular Location: Secreted, Extracellular matrix 

Purity: > 90% 

Traits: Freeze-dried powder 

Buffer formulation: 100mMNaHCO3, 500mMNaCl, pH8.3, containing 0.01% SKL, 5%Trehalose. 

Original Concentration: 250µg/mL 

Applications: Positive Control; Immunogen; SDS-PAGE; WB.(May be suitable for use in other assays to be determined by the end user.) 

Predicted isoelectric point: 3.7 

Predicted Molecular Mass: 54.6kDa 

Accurate Molecular Mass: 61kDa as determined by SDS-PAGE reducing conditions. 

Phenomenon explanation: 

The possible reasons that the actual band size differs from the predicted are as follows: 

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.

5.  Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ] 

Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex. 

[ STORAGE AND STABILITY ] 

 Storage: Avoid repeated freeze/thaw cycles. 

 Store at 2-8ºC for one month. 

 Aliquot and store at -80ºC for 12 months. 

Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.