Granzyme B, Mouse
概述
Synonyms | Granzyme B, Mouse |
Description | Granzyme B is a serine protease most commonly found in the granules of cytotoxic lymphocytes (CTLs), natural killer cells (NK cells) and cytotoxic T cells. It is secreted by these cells along with the pore forming protein perforin to mediate apoptosis in target cells. Granzyme B has also recently been found to be produced by a wide range of non-cytotoxic cells ranging from basophils and mast cells to smooth muscle cells. The secondary functions of granzyme B are also numerous. Granzyme B has been shown to be involved in inducing inflammation by stimulating cytokine release and is also involved in extracellular matrix remodeling. Recombinant Mouse Granzyme B produced in CHO cells is a polypeptide chain containing 227 amino acids. A fully biologically active molecule, rmGranzyme B has a molecular mass of 32 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript. |
Accession No | P04187 |
Species | Mouse |
Source | CHO |
Biological Activity | Measured by its ability to cleave a chromogenic peptide substrate (Ac-IEPD-pNA), the specific activity is greater than 1000 nM/min per μg of enzyme. |
Sequence | IIGGHEVKPH SRPYMALLSI KDQQPEAICG GFLIREDFVL |
特性
Measured Molecular Weight | 32 kDa, observed by reducing SDS-PAGE. |
Purity | > 98% as analyzed by SDS-PAGE. |
Formulation | Lyophilized after extensive dialysis against PBS. |
Reconstitution | Reconstituted in ddH2O or PBS at 100 μg/ml. |
Endotoxin Level | < 0.2 EU/μg, determined by LAL method. |
Storage | Lyophilized recombinant Mouse Granzyme B remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, recombinant Mouse Granzyme B should be stable up to 1 week at 4°C or up to 2 months at -20°C. |
Note | For research use only |