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NT-4, Mouse
概述 
| Synonyms | Neurotrophin-4, Neurotrophic 4/5 (NT-4/NT-5) |
| Description | Neurotrophin-4 (NT-4) is a small secreted cytokine, and belongs to the Neurotrophin (NT) family, which also includes Brain Derived Neurotropic Factor (BDNF), Nerve Growth Factor (NGF), and NT-3. NT family members are all derived from similar sized protein precursors, composed of N-terminal propeptides and C-terminal mature domains, which are separated by posttranslational proteolytic cleavage. NT-4 (along with NT-3) is foundin the brains of mammals . In vivo, NT-4 binds to the common receptor, p75NTR, and a tyrosine kinase receptor, TrkB. The heterotrimeric complex activates the NFκB transcription factor. NT-4 is essential for the differentiation and wiring regulation of the central and peripheral nervous systems during development, and is related to important diseases including Alzheimer’s. Recombinant mouse Neurotrophin-4 (rmNT-4) produced in E. coli is a noncovalently linked homodimer containing two non-glycosylated polypeptide chainsof 131 amino acids. A fully biologically active molecule, rmNT-4 has a molecular mass of 14.0kDaanalyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript. |
| Accession No | Q80VU4 |
| Species | Mouse |
| Source | E. coli |
| Biological Activity | ED50 <1µg/mL, measured by a cell proliferation assay usingC6cells, corresponding to a specific activity of>1× 103 units/mg. |
| Sequence | MGVSETAPAS RRGELAVCDA VSGWVTDRRT AVDLRGREVE |
特性
| Measured Molecular Weight | 14.0 kDa, observed by reducing SDS-PAGE. |
| Purity | > 95% by SDS-PAGEanalysis. |
| Formulation | Lyophilized after extensive dialysis against 50mM acetic acid. |
| Reconstitution | Reconstituted in 50mM acetic acid or ddH2O at 100 μg/mL. |
| Endotoxin Level | < 0.2 EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant mouse Neurotrophin-4 (rmNT-4) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rmNT-4 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C. |
| Note | For research use only |