ZP3 Polyclonal Antibody

Applications

Tested Applications:

IF, IHC, IP, WB, ELISA

Species specificity:

human, mouse, hamster

Positive WB detected in:

multi-cells

Positive IP detected in:

HeLa cells

Positive IHC detected in:

mouse ovary tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Positive IF detected in:

mouse ovary tissue

Recommended dilution:

WB : 1:500-1:1000

IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

IHC : 1:50-1:500

IF : 1:50-1:500

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:

ZP3 fusion protein Ag15791

Full name:

zona pellucida glycoprotein 3 (sperm receptor)

Calculated molecular weight:

373 aa, 41 kDa

Observed molecular weight:

47 kDa, 60 kDa

Gene symbol

ZP3

Synonyms

Sperm receptor, Zona pellucida glycoprotein 3, Zona pellucida protein C, Zp 3, ZP3, ZP3A, ZP3A/ZP3B, ZP3B, ZPC

Background

Zona pellucida (ZP) is an extracellular matrix that surrounds the oocyte and early embryo, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. Mammalian ZP consists of three to four glycoproteins, called ZP1, ZP2, ZP3, ZP4. ZP3 (zona pellucida glycoprotein 3, also known as zona pellucida sperm-binding protein 3) is essential for sperm binding and zona matrix formation. Human ZP3 gene encodes predicted precursor protein of 47 kDa that are processed into mature glycoprotein with larger apparent molecular mass